› 2y5w

X-ray diffraction
2.7Å resolution

Crystal structure of Drosophila melanogaster kinesin-1 motor domain dimer

Released:
DOI: 10.2210/pdb2y5w/pdb
PDB entry 2y5w coloured by chain, front view.
PDB entry 2y5w coloured by chain, side view.
PDB entry 2y5w coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The structure of the kinesin-1 motor-tail complex reveals the mechanism of autoinhibition.
Kaan HY, Hackney DD, Kozielski F
Science 333 883-5 (2011)
PMID: 21836017

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147897 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin heavy chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 40.89 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P17210 (Residues: 1-365; Coverage: 37%)
Gene names: CG7765, Khc, kin
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 76.89Å b: 89.18Å c: 138.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.21 0.289
Expression system: Escherichia coli BL21

Quick links

Citations

22 review citations

Axonal transport: cargo-specific mechanisms of motility and regulation.
Maday et al. (2014)
21 more

5 mentions without citation

Kinesin-2 motors: Kinetics and biophysics.
Gilbert et al. (2018)
4 more