› 2ybl

X-ray diffraction
2Å resolution

Nitrate X-ray induced reduction on HEWL crystals (17.9 MGy)

Released:
DOI: 10.2210/pdb2ybl/pdb
PDB entry 2ybl coloured by chain, front view.
PDB entry 2ybl coloured by chain, side view.
PDB entry 2ybl coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Effective scavenging at cryotemperatures: further increasing the dose tolerance of protein crystals.
De la Mora E, Carmichael I, Garman EF
J Synchrotron Radiat 18 346-57 (2011)
PMID: 21525642

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:
Ligand NO2 2 x NO2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P43212
Unit cell:
a: 78.1Å b: 78.1Å c: 38.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.253

Quick links

Citations

1 review citation

SAD phasing: History, current impact and future opportunities.
Rose et al. (2016)
14 other articles

2 mentions without citation

Protein structure determination by electron diffraction using a single three-dimensional nanocrystal.
Clabbers et al. (2017)
1 more