2yjl

X-ray diffraction
1.81Å resolution

Structural characterization of a secretin pilot protein from the type III secretion system (T3SS) of Pseudomonas aeruginosa

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-150899 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type 3 secretion system pilotin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 125 amino acids
Theoretical weight: 13.82 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26994 (Residues: 13-137; Coverage: 100%)
Gene names: PA1712, exsB
Sequence domains: Type III secretion system lipoprotein chaperone (YscW)
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 124.155Å b: 48.358Å c: 71.602Å
α: 90° β: 96.92° γ: 90°
R-values:
R R work R free
0.221 0.217 0.262
Expression system: Escherichia coli BL21(DE3)