› 2yjy

X-ray diffraction
2.6Å resolution

A specific and modular binding code for cytosine recognition in PUF domains

Released:
DOI: 10.2210/pdb2yjy/pdb
PDB entry 2yjy coloured by chain, front view.
PDB entry 2yjy coloured by chain, side view.
PDB entry 2yjy coloured by chain, top view.
Source organisms:
Primary publication:
Specific and modular binding code for cytosine recognition in Pumilio/FBF (PUF) RNA-binding domains.
Dong S, Wang Y, Cassidy-Amstutz C, Lu G, Bigler R, Jezyk MR, Li C, Hall TM, Wang Z
J Biol Chem 286 26732-42 (2011)
PMID: 21653694

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172065 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Pumilio homolog 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 40.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14671 (Residues: 828-1176; Coverage: 29%)
  • Best match: Q14671-2 (Residues: 802-1152)
Gene names: KIAA0099, PUM1, PUMH1
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
5'-R(*AP*UP*UP*GP*CP*AP*UP*AP*UP*AP)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 nucleotides
Theoretical weight: 3.15 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 35.796Å b: 59.34Å c: 344.724Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.21 0.306
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

20 review citations

Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.
Stynen et al. (2012)
19 more

6 mentions without citation

Engineering RNA-binding proteins for biology.
Chen et al. (2013)
5 more