› 2ysf

Solution NMR

Solution structure of the fourth WW domain from the human E3 ubiquitin-protein ligase Itchy homolog, ITCH

Released:
DOI: 10.2210/pdb2ysf/pdb
PDB entry 2ysf coloured by chain, ensemble of 20 models, front view.
PDB entry 2ysf coloured by chain, ensemble of 20 models, side view.
PDB entry 2ysf coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Entry authors: Ohnishi S, Li H, Koshiba S, Harada T, Watanabe S, Kigawa T, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-572412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Itchy homolog Chain: A
Molecule details ›
Chain: A
Length: 40 amino acids
Theoretical weight: 4.47 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96J02 (Residues: 480-512; Coverage: 4%)
Gene name: ITCH
Sequence domains: WW domain
Structure domains: Ubiquitin Ligase Nedd4; Chain: W;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Not provided

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