2ywj

X-ray diffraction
1.9Å resolution

Crystal structure of uncharacterized conserved protein from Methanocaldococcus jannaschii

Released:
Entry authors: Manzoku M, Ebihara A, Yokoyama S, Kuramitsu S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reactions catalysed:
L-glutamine + H(2)O = L-glutamate + NH(3)
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-561049 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyridoxal 5'-phosphate synthase subunit PdxT Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 20.63 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59055 (Residues: 1-186; Coverage: 100%)
Gene names: MJ1661, pdxT
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 44.816Å b: 50.47Å c: 70.404Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.213
Expression system: Escherichia coli