› 2z2i

X-ray diffraction
1.98Å resolution

Crystal structure of Peptidyl-tRNA hydrolase from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb2z2i/pdb
PDB entry 2z2i coloured by chain, front view.
PDB entry 2z2i coloured by chain, side view.
PDB entry 2z2i coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural plasticity and enzyme action: crystal structures of mycobacterium tuberculosis peptidyl-tRNA hydrolase.
Selvaraj M, Roy S, Singh NS, Sangeetha R, Varshney U, Vijayan M
J Mol Biol 372 186-93 (2007)
PMID: 17619020

Function and Biology Details

Reaction catalysed: 
N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidyl-tRNA hydrolase Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 20.49 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WHN7 (Residues: 1-191; Coverage: 100%)
Gene names: MTCY10G2.35, Rv1014c, pth
Sequence domains: Peptidyl-tRNA hydrolase
Structure domains: Peptidyl-tRNA hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 36.299Å b: 61.849Å c: 73.969Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.251
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Structural and functional insights into peptidyl-tRNA hydrolase.
Sharma et al. (2014)
28 other articles

9 mentions without citation

NMR structure determination for larger proteins using backbone-only data.
Raman et al. (2010)
8 more