› 2z48

X-ray diffraction
1.7Å resolution

Crystal Structure of Hemolytic Lectin CEL-III Complexed with GalNac

Released:
DOI: 10.2210/pdb2z48/pdb
PDB entry 2z48 coloured by chain, front view.
PDB entry 2z48 coloured by chain, side view.
PDB entry 2z48 coloured by chain, top view.
Source organism: Cucumaria echinata
Primary publication:
C-type lectin-like carbohydrate recognition of the hemolytic lectin CEL-III containing ricin-type -trefoil folds.
Hatakeyama T, Unno H, Kouzuma Y, Uchida T, Eto S, Hidemura H, Kato N, Yonekura M, Kusunoki M
J Biol Chem 282 37826-35 (2007)
PMID: 17977832

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183107 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose/N-acetylgalactosamine-binding lectin CEL-III Chains: A, B
Molecule details ›
Chains: A, B
Length: 432 amino acids
Theoretical weight: 47.45 KDa
Source organism: Cucumaria echinata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q868M7 (Residues: 11-442; Coverage: 98%)
Gene name: cel3
Sequence domains: Ricin-type beta-trefoil lectin domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand A2G 4 x A2G
Ligand NGA 8 x NGA
Ligand CA 10 x CA
Ligand MG 4 x MG
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P1
Unit cell:
a: 52.637Å b: 65.169Å c: 66.675Å
α: 85.3° β: 73.55° γ: 89.94°
R-values:
R R work R free
0.186 0.185 0.218
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Sugar recognition and protein-protein interaction of mammalian lectins conferring diverse functions.
Nagae et al. (2015)
17 other articles

4 mentions without citation

Functional Diversity of Novel Lectins with Unique Structural Features in Marine Animals.
Hatakeyama et al. (2023)
3 more