› 2z5b

X-ray diffraction
1.96Å resolution

Crystal Structure of a Novel Chaperone Complex for Yeast 20S Proteasome Assembly

Released:
DOI: 10.2210/pdb2z5b/pdb
PDB entry 2z5b coloured by chain, front view.
PDB entry 2z5b coloured by chain, side view.
PDB entry 2z5b coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes.
Yashiroda H, Mizushima T, Okamoto K, Kameyama T, Hayashi H, Kishimoto T, Niwa S, Kasahara M, Kurimoto E, Sakata E, Takagi K, Suzuki A, Hirano Y, Murata S, Kato K, Yamane T, Tanaka K
Nat Struct Mol Biol 15 228-36 (2008)
PMID: 18278057

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170570 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proteasome chaperone 4 Chain: A
Molecule details ›
Chain: A
Length: 151 amino acids
Theoretical weight: 16.88 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12245 (Residues: 1-148; Coverage: 100%)
Gene names: DMP1, POC4, YPL144W
Sequence domains: 20S proteasome chaperone assembly proteins 3 and 4
Structure domains: Ribosomal Protein S5; domain 2
Proteasome chaperone 3 Chain: B
Molecule details ›
Chain: B
Length: 179 amino acids
Theoretical weight: 20.08 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07951 (Residues: 1-179; Coverage: 100%)
Gene names: DMP2, IRC25, POC3, YLR021W
Sequence domains: 20S proteasome chaperone assembly proteins 3 and 4
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P31
Unit cell:
a: 57.487Å b: 57.487Å c: 82.206Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.24 0.287
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

30 review citations

The proteasome: overview of structure and functions.
Tanaka K. (2009)
29 more

4 mentions without citation

Molecular and Structural Basis of the Proteasome α Subunit Assembly Mechanism Mediated by the Proteasome-Assembling Chaperone PAC3-PAC4 Heterodimer.
Satoh et al. (2019)
3 more