› 2z75

X-ray diffraction
1.7Å resolution

T. tengcongensis glmS ribozyme bound to glucosamine-6-phosphate

Released:
DOI: 10.2210/pdb2z75/pdb
PDB entry 2z75 coloured by chain, front view.
PDB entry 2z75 coloured by chain, side view.
PDB entry 2z75 coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Primary publication:
Requirement of helix P2.2 and nucleotide G1 for positioning the cleavage site and cofactor of the glmS ribozyme.
Klein DJ, Wilkinson SR, Been MD, Ferré-D'Amaré AR
J Mol Biol 373 178-89 (2007)
PMID: 17804015

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-249586 (preferred)
Entry contents:
1 distinct RNA molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
glmS ribozyme RNA Chain: B
Molecule details ›
Chain: B
Length: 125 nucleotides
Theoretical weight: 40.54 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Expression system: Not provided
Sequence domains: glmS glucosamine-6-phosphate activated ribozyme aptamer
glmS ribozyme substrate RNA Chain: A
Molecule details ›
Chain: A
Length: 27 nucleotides
Theoretical weight: 8.59 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand GLP 1 x GLP
Ligand MG 9 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21212
Unit cell:
a: 180.935Å b: 39.942Å c: 70.435Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.239
Expression system: Not provided

Quick links

Citations

15 review citations

The structural and functional diversity of metabolite-binding riboswitches.
Roth et al. (2009)
14 more

21 mentions without citation

Long-Range Interactions in Riboswitch Control of Gene Expression.
Jones et al. (2017)
20 more