› 2zqj

X-ray diffraction
2.9Å resolution

Substrate-Free Form of Cytochrome P450BSbeta

Released:
DOI: 10.2210/pdb2zqj/pdb
PDB entry 2zqj coloured by chain, front view.
PDB entry 2zqj coloured by chain, side view.
PDB entry 2zqj coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Understanding substrate misrecognition of hydrogen peroxide dependent cytochrome P450 from Bacillus subtilis.
Shoji O, Fujishiro T, Nagano S, Tanaka S, Hirose T, Shiro Y, Watanabe Y
J Biol Inorg Chem 15 1331-9 (2010)
PMID: 20697922

Function and Biology Details

Reaction catalysed: 
Fatty acid + H(2)O(2) = 3- or 2-hydroxy fatty acid + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty-acid peroxygenase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 417 amino acids
Theoretical weight: 48.18 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O31440 (Residues: 1-417; Coverage: 100%)
Gene names: BSU02100, CYP152A1, cypC
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 3 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: R32
Unit cell:
a: 173.076Å b: 173.076Å c: 279.616Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.221 0.286
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Diversity of P450 enzymes in the biosynthesis of natural products.
Podust et al. (2012)
4 more

3 mentions without citation

Structure and biochemical properties of the alkene producing cytochrome P450 OleTJE (CYP152L1) from the Jeotgalicoccus sp. 8456 bacterium.
Belcher et al. (2014)
2 more