› 2zxp

X-ray diffraction
2.3Å resolution

Crystal structure of RecJ in complex with Mn2+ from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2zxp/pdb
PDB entry 2zxp coloured by chain, front view.
PDB entry 2zxp coloured by chain, side view.
PDB entry 2zxp coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structure of RecJ exonuclease defines its specificity for single-stranded DNA.
Wakamatsu T, Kitamura Y, Kotera Y, Nakagawa N, Kuramitsu S, Masui R
J Biol Chem 285 9762-9769 (2010)
PMID: 20129927

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-562216 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Single-stranded-DNA-specific exonuclease RecJ Chain: A
Molecule details ›
Chain: A
Length: 666 amino acids
Theoretical weight: 72.96 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SJ47 (Residues: 1-666; Coverage: 100%)
Gene names: TTHA1167, recJ
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P43212
Unit cell:
a: 83.127Å b: 83.127Å c: 249.937Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.281
Expression system: Escherichia coli

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Citations

2 review citations

Structure and evolutionary origins of the CMG complex.
Onesti et al. (2013)
1 more

11 mentions without citation

Structure of RecJ exonuclease defines its specificity for single-stranded DNA.
Wakamatsu et al. (2010)
10 more