› 3ank

X-ray diffraction
2.02Å resolution

Crystal structure of unsaturated glucuronyl hydrolase mutant D175N from Streptcoccus agalactiae complexed with dGlcA-GalNAc6S

Released:
DOI: 10.2210/pdb3ank/pdb
PDB entry 3ank coloured by chain, front view.
PDB entry 3ank coloured by chain, side view.
PDB entry 3ank coloured by chain, top view.
Source organism: Streptococcus agalactiae serogroup III
Primary publication:
Structural determinants in streptococcal unsaturated glucuronyl hydrolase for recognition of glycosaminoglycan sulfate groups.
Nakamichi Y, Maruyama Y, Mikami B, Hashimoto W, Murata K
J Biol Chem 286 6262-71 (2011)
PMID: 21147778

Function and Biology Details

Reaction catalysed: 
Beta-D-4-deoxy-Delta(4)-GlcAp-(1->3)-beta-D-GalNAc6S + H(2)O = 5-dehydro-4-deoxy-D-glucuronate + N-acetyl-beta-D-galactosamine-6-O-sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-184296 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Unsaturated chondroitin disaccharide hydrolase Chain: A
Molecule details ›
Chain: A
Length: 398 amino acids
Theoretical weight: 46.64 KDa
Source organism: Streptococcus agalactiae serogroup III
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8E372 (Residues: 1-398; Coverage: 100%)
Gene name: gbs1889
Sequence domains: Glycosyl Hydrolase Family 88
Structure domains: Glycosyltransferase

Ligands and Environments

Carbohydrate polymer : NEW Components: NG6, GCD
Carbohydrate polymer
1 bound ligand:
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: C2
Unit cell:
a: 105.178Å b: 53.267Å c: 70.136Å
α: 90° β: 96.58° γ: 90°
R-values:
R R work R free
0.176 0.174 0.209
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Metabolic fate of unsaturated glucuronic/iduronic acids from glycosaminoglycans: molecular identification and structure determination of streptococcal isomerase and dehydrogenase.
Maruyama et al. (2015)
7 more

9 mentions without citation

The ankyrin repeat as molecular architecture for protein recognition.
Mosavi et al. (2004)
8 more