› 3ask

X-ray diffraction
2.9Å resolution

Structure of UHRF1 in complex with histone tail

Released:
DOI: 10.2210/pdb3ask/pdb
PDB entry 3ask coloured by chain, front view.
PDB entry 3ask coloured by chain, side view.
PDB entry 3ask coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Recognition of modification status on a histone H3 tail by linked histone reader modules of the epigenetic regulator UHRF1.
Arita K, Isogai S, Oda T, Unoki M, Sugita K, Sekiyama N, Kuwata K, Hamamoto R, Tochio H, Sato M, Ariyoshi M, Shirakawa M
Proc Natl Acad Sci U S A 109 12950-5 (2012)
PMID: 22837395

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-160866 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase UHRF1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 226 amino acids
Theoretical weight: 26.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96T88 (Residues: 134-367; Coverage: 28%)
Gene names: ICBP90, NP95, RNF106, UHRF1
Sequence domains:
Structure domains:
Histone H3.3 Chains: P, Q, R
Molecule details ›
Chains: P, Q, R
Length: 13 amino acids
Theoretical weight: 1.41 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P84243 (Residues: 2-14; Coverage: 10%)
Gene names: H3-3A, H3-3B, H3.3A, H3.3B, H3F3, H3F3A, H3F3B, PP781

Ligands and Environments

1 bound ligand:
Ligand ZN 7 x ZN
1 modified residue:
Ligand M3L 3 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P42212
Unit cell:
a: 145.178Å b: 145.178Å c: 125.413Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.244 0.286
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

42 review citations

Perceiving the epigenetic landscape through histone readers.
Musselman et al. (2012)
41 more

16 mentions without citation

Readout of epigenetic modifications.
Patel et al. (2013)
15 more