› 3bp4

X-ray diffraction
1.85Å resolution

The high resolution crystal structure of HLA-B*2705 in complex with a Cathepsin A signal sequence peptide pCatA

Released:
DOI: 10.2210/pdb3bp4/pdb
PDB entry 3bp4 coloured by chain, front view.
PDB entry 3bp4 coloured by chain, side view.
PDB entry 3bp4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for T cell alloreactivity among three HLA-B14 and HLA-B27 antigens.
Kumar P, Vahedi-Faridi A, Saenger W, Merino E, López de Castro JA, Uchanska-Ziegler B, Ziegler A
J Biol Chem 284 29784-97 (2009)
PMID: 19617632

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal amino acid with broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135149 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01889 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Lysosomal protective protein Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 982 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P10619 (Residues: 2-10; Coverage: 0%)
Gene names: CTSA, PPGB

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 51.161Å b: 82.4Å c: 109.846Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.222
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Recent advances in Takayasu arteritis.
Terao et al. (2014)
4 more

8 mentions without citation

The immune-related role of beta-2-microglobulin in melanoma.
Wang et al. (2022)
7 more