› 3bwh

X-ray diffraction
1Å resolution

Atomic resolution structure of cucurmosin, a novel type 1 RIP from the sarcocarp of Cucurbita moschata

Released:
DOI: 10.2210/pdb3bwh/pdb
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PDB entry 3bwh coloured by chain, top view.
Source organism: Cucurbita moschata
Primary publication:
Atomic resolution structure of cucurmosin, a novel type 1 ribosome-inactivating protein from the sarcocarp of Cucurbita moschata.
Hou X, Meehan EJ, Xie J, Huang M, Chen M, Chen L
J Struct Biol 164 81-7 (2008)
PMID: 18652900

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112085 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
rRNA N-glycosylase Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 27.1 KDa
Source organism: Cucurbita moschata
UniProt:
  • Canonical: D0VWS7 (Residues: 1-245; Coverage: 100%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, XYP, MAN
Carbohydrate polymer
2 bound ligands:
Ligand PO4 1 x PO4
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 41.763Å b: 58.782Å c: 99.523Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.179

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Citations

6 review citations

Medicinal and biological potential of pumpkin: an updated review.
Yadav et al. (2010)
5 more

1 mention without citation

Structures and Ribosomal Interaction of Ribosome-Inactivating Proteins.
Shi et al. (2016)

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