› 3c5h

X-ray diffraction
1.8Å resolution

Crystal structure of the Ras homolog domain of human GRLF1 (p190RhoGAP)

Released:
DOI: 10.2210/pdb3c5h/pdb
PDB entry 3c5h coloured by chain, front view.
PDB entry 3c5h coloured by chain, side view.
PDB entry 3c5h coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Shen L, Tong Y, Tempel W, MacKenzie F, Arrowsmith CH, Edwards AM, Bountra C, Weigelt J, Bochkarev A, Park H, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192576 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho GTPase-activating protein 35 Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 28.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NRY4 (Residues: 13-249; Coverage: 16%)
Gene names: ARHGAP35, GRF1, GRLF1, KIAA1722, P190A, p190ARHOGAP
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand GNP 1 x GNP
Ligand UNX 5 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B, APS BEAMLINE 19-ID
Spacegroup: I4
Unit cell:
a: 108.959Å b: 108.959Å c: 51.319Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.247
Expression system: Escherichia coli

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