› 3czt

X-ray diffraction
1.4Å resolution

Crystal Structure of S100B in the Calcium and Zinc Loaded State at pH 9

Released:
DOI: 10.2210/pdb3czt/pdb
PDB entry 3czt coloured by chain, front view.
PDB entry 3czt coloured by chain, side view.
PDB entry 3czt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structures of human S100B in the zinc- and calcium-loaded state at three pH values reveal zinc ligand swapping.
Ostendorp T, Diez J, Heizmann CW, Fritz G
Biochim Biophys Acta 1813 1083-91 (2011)
PMID: 20950652

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein S100-B Chain: X
Molecule details ›
Chain: X
Length: 92 amino acids
Theoretical weight: 10.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04271 (Residues: 1-92; Coverage: 100%)
Gene name: S100B
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 34.98Å b: 89.26Å c: 59.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Functions of S100 proteins.
Donato et al. (2013)
5 more

2 mentions without citation

Multiple Evolutionary Origins of Ubiquitous Cu2+ and Zn2+ Binding in the S100 Protein Family.
Wheeler et al. (2016)
1 more