› 3d1n

X-ray diffraction
2.51Å resolution

Structure of human Brn-5 transcription factor in complex with corticotrophin-releasing hormone gene promoter

Released:
DOI: 10.2210/pdb3d1n/pdb
PDB entry 3d1n coloured by chain, front view.
PDB entry 3d1n coloured by chain, side view.
PDB entry 3d1n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human Brn-5 transcription factor in complex with CRH gene promoter.
Pereira JH, Kim SH
J Struct Biol 167 159-65 (2009)
PMID: 19450691

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116167 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
POU domain, class 6, transcription factor 1 Chains: I, J, K, L, M, N, O, P
Molecule details ›
Chains: I, J, K, L, M, N, O, P
Length: 151 amino acids
Theoretical weight: 17.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14863 (Residues: 142-292; Coverage: 50%)
Gene names: BRN5, MPOU, POU6F1, TCFB1
Sequence domains:
Structure domains:
5'-D(*DAP*DGP*DCP*DAP*DTP*DAP*DAP*DAP*DTP*DAP*DAP*DTP*DAP*DA)-3' Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 14 nucleotides
Theoretical weight: 4.3 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*DTP*DTP*DAP*DTP*DTP*DAP*DTP*DTP*DTP*DAP*DTP*DGP*DCP*DT)-3' Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 14 nucleotides
Theoretical weight: 4.25 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 100.298Å b: 112.06Å c: 181.504Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.27
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

OCT4: dynamic DNA binding pioneers stem cell pluripotency.
Jerabek et al. (2014)
1 more

7 mentions without citation

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
6 more