› 3djg

X-ray diffraction
1.8Å resolution

Catalytic cycle of human glutathione reductase near 1 A resolution

Released:
DOI: 10.2210/pdb3djg/pdb
PDB entry 3djg coloured by chain, front view.
PDB entry 3djg coloured by chain, side view.
PDB entry 3djg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Catalytic cycle of human glutathione reductase near 1 A resolution.
Berkholz DS, Faber HR, Savvides SN, Karplus PA
J Mol Biol 382 371-84 (2008)
PMID: 18638483

Function and Biology Details

Reaction catalysed: 
2 glutathione + NADP(+) = glutathione disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione reductase, mitochondrial Chain: X
Molecule details ›
Chain: X
Length: 477 amino acids
Theoretical weight: 51.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00390 (Residues: 45-522; Coverage: 91%)
Gene names: GLUR, GRD1, GSR
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand NDP 1 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2
Unit cell:
a: 119.532Å b: 63.362Å c: 84.619Å
α: 90° β: 121.54° γ: 90°
R-values:
R R work R free
0.145 0.141 0.186
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Glutathione catalysis and the reaction mechanisms of glutathione-dependent enzymes.
Deponte M. (2013)
11 more

4 mentions without citation

Catalytic cycle of human glutathione reductase near 1 A resolution.
Berkholz et al. (2008)
3 more