› 3dqf

X-ray diffraction
1.46Å resolution

Structure of the Yellow Fluorescent Protein Citrine Frozen at 1000 Atmospheres Number 6: Structure 10 in a Series of 26 High Pressure Structures

Released:
DOI: 10.2210/pdb3dqf/pdb
PDB entry 3dqf coloured by chain, front view.
PDB entry 3dqf coloured by chain, side view.
PDB entry 3dqf coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Alteration of citrine structure by hydrostatic pressure explains the accompanying spectral shift.
Barstow B, Ando N, Kim CU, Gruner SM
Proc Natl Acad Sci U S A 105 13362-6 (2008)
PMID: 18768811

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 27.39 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CR2 1 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 51.45Å b: 62.272Å c: 70.366Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.244
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

Design and development of genetically encoded fluorescent sensors to monitor intracellular chemical and physical parameters.
Germond et al. (2016)
8 more