› 3fdo

X-ray diffraction
1.4Å resolution

Structure of human MDMX in complex with high affinity peptide

Released:
DOI: 10.2210/pdb3fdo/pdb
PDB entry 3fdo coloured by chain, front view.
PDB entry 3fdo coloured by chain, side view.
PDB entry 3fdo coloured by chain, top view.
Source organisms:
Primary publication:
High affinity interaction of the p53 peptide-analogue with human Mdm2 and Mdmx.
Czarna A, Popowicz GM, Pecak A, Wolf S, Dubin G, Holak TA
Cell Cycle 8 1176-84 (2009)
PMID: 19305137

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127331 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein Mdm4 Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 10.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O15151 (Residues: 23-111; Coverage: 18%)
Gene names: MDM4, MDMX
Structure domains: SWIB/MDM2 domain
Synthetic high affinity peptide Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.5 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 69.23Å b: 30.95Å c: 50.42Å
α: 90° β: 124.41° γ: 90°
R-values:
R R work R free
0.188 0.179 0.247
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

4 review citations

Awakening guardian angels: drugging the p53 pathway.
Brown et al. (2009)
3 more

4 mentions without citation

Synthesis of cell-permeable stapled peptide dual inhibitors of the p53-Mdm2/Mdmx interactions via photoinduced cycloaddition.
Madden et al. (2011)
3 more