› 3frr

X-ray diffraction
1.8Å resolution

Structure of human IST1(NTD) - (residues 1-189)(P21)

Released:
DOI: 10.2210/pdb3frr/pdb
PDB entry 3frr coloured by chain, front view.
PDB entry 3frr coloured by chain, side view.
PDB entry 3frr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for ESCRT-III protein autoinhibition.
Bajorek M, Schubert HL, McCullough J, Langelier C, Eckert DM, Stubblefield WM, Uter NT, Myszka DG, Hill CP, Sundquist WI
OpenAccess logo Nat Struct Mol Biol 16 754-62 (2009)
PMID: 19525971

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156916 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
IST1 homolog Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 21.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53990 (Residues: 1-189; Coverage: 52%)
Gene names: IST1, KIAA0174
Sequence domains: Regulator of Vps4 activity in the MVB pathway
Structure domains: Vacuolar protein sorting-associated protein Ist1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 29.251Å b: 92.364Å c: 33.251Å
α: 90° β: 97.02° γ: 90°
R-values:
R R work R free
0.197 0.195 0.247
Expression system: Escherichia coli

Quick links

Citations

40 review citations

The ESCRT pathway.
Henne et al. (2011)
39 more

9 mentions without citation

The ESCRT machinery: from the plasma membrane to endosomes and back again.
Schuh et al. (2014)
8 more