› 3fvj

X-ray diffraction
2.3Å resolution

Crystal structure of phospholipase A2 1B crystallized in the presence of octyl sulfate

Released:
DOI: 10.2210/pdb3fvj/pdb
PDB entry 3fvj coloured by chain, front view.
PDB entry 3fvj coloured by chain, side view.
PDB entry 3fvj coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structure of a premicellar complex of alkyl sulfates with the interfacial binding surfaces of four subunits of phospholipase A2.
Pan YH, Bahnson BJ
Biochim Biophys Acta 1804 1443-8 (2010)
PMID: 20302975

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2, major isoenzyme Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 14.01 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00592 (Residues: 23-146; Coverage: 95%)
Gene name: PLA2G1B
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P3121
Unit cell:
a: 69.064Å b: 69.064Å c: 68.84Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.201 0.249
Expression system: Escherichia coli

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Citations

2 review citations

Phospholipase A2 enzymes: physical structure, biological function, disease implication, chemical inhibition, and therapeutic intervention.
Dennis et al. (2011)
1 more

1 mention without citation

Structure of a premicellar complex of alkyl sulfates with the interfacial binding surfaces of four subunits of phospholipase A2.
Pan et al. (2010)