› 3fw7

X-ray diffraction
1.82Å resolution

Structure of berberine bridge enzyme, H104A variant

Released:
DOI: 10.2210/pdb3fw7/pdb
PDB entry 3fw7 coloured by chain, front view.
PDB entry 3fw7 coloured by chain, side view.
PDB entry 3fw7 coloured by chain, top view.
Source organism: Eschscholzia californica
Primary publication:
Structural roles of biocovalent flaninylation in berberine bridge enzyme
Winkler A, Motz K, Riedl S, Puhl M, Macheroux P, Gruber K
To be published

Function and Biology Details

Reaction catalysed: 
(S)-reticuline + O(2) = (S)-scoulerine + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151854 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Reticuline oxidase Chain: A
Molecule details ›
Chain: A
Length: 498 amino acids
Theoretical weight: 55.54 KDa
Source organism: Eschscholzia californica
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P30986 (Residues: 24-520; Coverage: 97%)
Gene name: BBE1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 68.8Å b: 68.8Å c: 247.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.194
Expression system: Komagataella pastoris

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