› 3gut

X-ray diffraction
3.59Å resolution

Crystal structure of a higher-order complex of p50:RelA bound to the HIV-1 LTR

Released:
DOI: 10.2210/pdb3gut/pdb
PDB entry 3gut coloured by chain, front view.
PDB entry 3gut coloured by chain, side view.
PDB entry 3gut coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of HIV-1 activation by NF-kappaB--a higher-order complex of p50:RelA bound to the HIV-1 LTR.
Stroud JC, Oltman A, Han A, Bates DL, Chen L
J Mol Biol 393 98-112 (2009)
PMID: 19683540

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-507512 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Transcription factor p65 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 273 amino acids
Theoretical weight: 30.98 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q04206 (Residues: 20-291; Coverage: 49%)
Gene names: NFKB3, RELA
Sequence domains:
Structure domains:
Nuclear factor NF-kappa-B p50 subunit Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 312 amino acids
Theoretical weight: 34.92 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P19838 (Residues: 41-352; Coverage: 32%)
Gene name: NFKB1
Sequence domains:
Structure domains:
HIV-LTR Core Forward Strand Chains: I, X
Molecule details ›
Chains: I, X
Length: 26 nucleotides
Theoretical weight: 8 KDa
HIV-LTR Core Reverse Strand Chains: J, Y
Molecule details ›
Chains: J, Y
Length: 26 nucleotides
Theoretical weight: 7.98 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P42
Unit cell:
a: 167.493Å b: 167.493Å c: 172.757Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.301

Quick links

Citations

12 review citations

The molecular biology of HIV latency: breaking and restoring the Tat-dependent transcriptional circuit.
Karn J. (2011)
11 more

18 mentions without citation

Structural basis of HIV-1 activation by NF-kappaB--a higher-order complex of p50:RelA bound to the HIV-1 LTR.
Stroud et al. (2009)
17 more