› 3h4f

X-ray diffraction
2.1Å resolution

Met62Leu variant of nitrite reductase from Alcaligenes faeclis

Released:
DOI: 10.2210/pdb3h4f/pdb
PDB entry 3h4f coloured by chain, front view.
PDB entry 3h4f coloured by chain, side view.
PDB entry 3h4f coloured by chain, top view.
Source organism: Alcaligenes faecalis
Primary publication:
Directed evolution of copper nitrite reductase to a chromogenic reductant.
MacPherson IS, Rosell FI, Scofield M, Mauk AG, Murphy ME
Protein Eng Des Sel 23 137-45 (2010)
PMID: 20083495

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 336 amino acids
Theoretical weight: 36.3 KDa
Source organism: Alcaligenes faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38501 (Residues: 40-375; Coverage: 98%)
Gene names: nir, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 6 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 61.132Å b: 102.223Å c: 146.145Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.166 0.227
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Biocatalyst development by directed evolution.
Wang et al. (2012)
15 other articles

1 mention without citation

Directed evolution of copper nitrite reductase to a chromogenic reductant.
MacPherson et al. (2010)