3hu6

X-ray diffraction
2.7Å resolution

Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases: SPOPMATHx/BTB/3-box-PucSBC1

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-129128 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Speckle-type POZ protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 35.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43791 (Residues: 28-329; Coverage: 81%)
Gene name: SPOP
Sequence domains:
Structure domains:
Puckered, isoform A Chains: C, D
Molecule details ›
Chains: C, D
Length: 7 amino acids
Theoretical weight: 752 Da
Source organism: Drosophila melanogaster
Expression system: Not provided
UniProt:
  • Canonical: Q9VHV8 (Residues: 96-102; Coverage: 2%)
Gene names: 0238/03, 1351/08, CG7850, Dmel\CG7850, Dmel_CG7850, JNK-DSP, PUC, PUC/MKP, Puc, dPuc, hrt, l(3)84Eh, l(3)A251.1, l(3)j4E1, puc, vco

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 63.74Å b: 107.74Å c: 130.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.24 0.296
Expression systems:
  • Escherichia coli
  • Not provided