› 3iid

X-ray diffraction
1.9Å resolution

Crystal structure of the macro domain of human histone macroH2A1.1 in complex with ADP-ribose (form A)

Released:
DOI: 10.2210/pdb3iid/pdb
PDB entry 3iid coloured by chain, front view.
PDB entry 3iid coloured by chain, side view.
PDB entry 3iid coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A macrodomain-containing histone rearranges chromatin upon sensing PARP1 activation.
Timinszky G, Till S, Hassa PO, Hothorn M, Kustatscher G, Nijmeijer B, Colombelli J, Altmeyer M, Stelzer EH, Scheffzek K, Hottiger MO, Ladurner AG
Nat Struct Mol Biol 16 923-9 (2009)
PMID: 19680243

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Core histone macro-H2A.1 Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 22.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75367 (Residues: 162-369; Coverage: 56%)
Gene names: H2AFY, MACROH2A1
Sequence domains: Macro domain
Structure domains: Leucine Aminopeptidase, subunit E, domain 1

Ligands and Environments

2 bound ligands:
Ligand APR 1 x APR
Ligand NO3 1 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P61
Unit cell:
a: 86.26Å b: 86.26Å c: 51.04Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.176 0.215
Expression system: Escherichia coli

Quick links

Citations

114 review citations

The DNA damage response: making it safe to play with knives.
Ciccia et al. (2010)
113 more

8 mentions without citation

The macro domain protein family: structure, functions, and their potential therapeutic implications.
Han et al. (2011)
7 more