› 3in0

X-ray diffraction
2.35Å resolution

Crystal structure of the F114P/M121Q variant of Pseudomonas aeruginosa azurin in the Cu(II) state

Released:
DOI: 10.2210/pdb3in0/pdb
PDB entry 3in0 coloured by chain, front view.
PDB entry 3in0 coloured by chain, side view.
PDB entry 3in0 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Rationally tuning the reduction potential of a single cupredoxin beyond the natural range.
Marshall NM, Garner DK, Wilson TD, Gao YG, Robinson H, Nilges MJ, Lu Y
Nature 462 113-6 (2009)
PMID: 19890331

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 128 amino acids
Theoretical weight: 13.91 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 4 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 44.26Å b: 49.59Å c: 60.51Å
α: 100.39° β: 103.42° γ: 104.31°
R-values:
R R work R free
0.253 0.246 0.286
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Copper active sites in biology.
Solomon et al. (2014)
21 more

3 mentions without citation

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
2 more