› 3ipm

X-ray diffraction
4Å resolution

Crystal Structure of Archaeal 20S Proteasome in Complex with the C-terminus of PAN

Released:
DOI: 10.2210/pdb3ipm/pdb
PDB entry 3ipm coloured by chain, front view.
PDB entry 3ipm coloured by chain, side view.
PDB entry 3ipm coloured by chain, top view.
Source organisms:
Primary publication:
Interactions of PAN's C-termini with archaeal 20S proteasome and implications for the eukaryotic proteasome-ATPase interactions.
Yu Y, Smith DM, Kim HM, Rodriguez V, Goldberg AL, Cheng Y
EMBO J 29 692-702 (2010)
PMID: 20019667

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 42-mer (preferred)
PDBe Complex ID:
PDB-CPX-150392 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proteasome subunit alpha Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 233 amino acids
Theoretical weight: 25.83 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25156 (Residues: 1-233; Coverage: 100%)
Gene names: Ta1288, psmA
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta Chains: H, I, J, K, L, M, N
Molecule details ›
Chains: H, I, J, K, L, M, N
Length: 217 amino acids
Theoretical weight: 24 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28061 (Residues: 1-211; Coverage: 100%)
Gene names: Ta0612, psmB
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome activator PA28 C-terminal domain-containing protein; Proteasome-activating nucleotidase Chains: O, P, Q, R, S, T, U
Molecule details ›
Chains: O, P, Q, R, S, T, U
Length: 239 amino acids
Theoretical weight: 26.33 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q38BM8 (Residues: 2-223; Coverage: 96%)
  • Canonical: Q58576 (Residues: 424-430; Coverage: 2%)
Gene names: MJ1176, Tb10.70.3660, pan
Sequence domains: Proteasome activator PA28, C-terminal
Structure domains: Proteasome activator pa28, C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P4222
Unit cell:
a: 166.89Å b: 166.89Å c: 412.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.249 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

22 review citations

The Logic of the 26S Proteasome.
Collins et al. (2017)
21 more

13 mentions without citation

Molecular architecture and assembly of the eukaryotic proteasome.
Tomko et al. (2013)
12 more