› 3kkm

X-ray diffraction
1.7Å resolution

Crystal structure of H-Ras T35S in complex with GppNHp

Released:
DOI: 10.2210/pdb3kkm/pdb
PDB entry 3kkm coloured by chain, front view.
PDB entry 3kkm coloured by chain, side view.
PDB entry 3kkm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for conformational dynamics of GTP-bound Ras protein.
Shima F, Ijiri Y, Muraoka S, Liao J, Ye M, Araki M, Matsumoto K, Yamamoto N, Sugimoto T, Yoshikawa Y, Kumasaka T, Yamamoto M, Tamura A, Kataoka T
J Biol Chem 285 22696-705 (2010)
PMID: 20479006

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 172 amino acids
Theoretical weight: 19.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GNP 1 x GNP
Ligand MG 1 x MG
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: R32
Unit cell:
a: 92.855Å b: 92.855Å c: 117.478Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.195 0.232
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Direct small-molecule inhibitors of KRAS: from structural insights to mechanism-based design.
Ostrem et al. (2016)
18 more

6 mentions without citation

Structural basis for conformational dynamics of GTP-bound Ras protein.
Shima et al. (2010)
5 more