› 3kn5

X-ray diffraction
2.4Å resolution

Crystal structure of the C-terminal kinase domain of msk1 in complex with AMP-PNP

Released:
DOI: 10.2210/pdb3kn5/pdb
PDB entry 3kn5 coloured by chain, front view.
PDB entry 3kn5 coloured by chain, side view.
PDB entry 3kn5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of the active form of the C-terminal kinase domain of mitogen- and stress-activated protein kinase 1.
Malakhova M, D'Angelo I, Kim HG, Kurinov I, Bode AM, Dong Z
J Mol Biol 399 41-52 (2010)
PMID: 20382163

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal protein S6 kinase alpha-5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 325 amino acids
Theoretical weight: 37.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75582 (Residues: 414-738; Coverage: 41%)
Gene names: MSK1, RPS6KA5
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 51.561Å b: 91.25Å c: 135.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.278
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Yersinia effector YopO uses actin as bait to phosphorylate proteins that regulate actin polymerization.
Lee et al. (2015)
2 more

7 mentions without citation

Disordered Protein Kinase Regions in Regulation of Kinase Domain Cores.
Gógl et al. (2019)
6 more