› 3kwi

X-ray diffraction
2.21Å resolution

X-ray structure of NS1 effector domain W187Y mutant

Released:
DOI: 10.2210/pdb3kwi/pdb
PDB entry 3kwi coloured by chain, front view.
PDB entry 3kwi coloured by chain, side view.
PDB entry 3kwi coloured by chain, top view.
Source organism: Influenza A virus (A/Udorn/307/1972(H3N2))
Primary publication:
X-ray structures of NS1 effector domain mutants.
Xia S, Robertus JD
Arch Biochem Biophys 494 198-204 (2010)
PMID: 19995550

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-527756 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-structural protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 141 amino acids
Theoretical weight: 16.01 KDa
Source organism: Influenza A virus (A/Udorn/307/1972(H3N2))
Expression system: Escherichia coli
UniProt:
  • Canonical: P03495 (Residues: 74-74, 78-205; Coverage: 54%)
Gene name: NS
Sequence domains: Influenza non-structural protein (NS1)
Structure domains: Influenza virus non-structural protein, effector domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 47.991Å b: 60.236Å c: 132.655Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.258
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Conformational plasticity of the influenza A virus NS1 protein.
Hale BG. (2014)
4 more

2 mentions without citation

X-ray structures of NS1 effector domain mutants.
Xia et al. (2010)
1 more