› 3kxf

X-ray diffraction
3.1Å resolution

Crystal Structure of SB27 TCR in complex with the 'restriction triad' mutant HLA-B*3508-13mer

Released:
DOI: 10.2210/pdb3kxf/pdb
PDB entry 3kxf coloured by chain, front view.
PDB entry 3kxf coloured by chain, side view.
PDB entry 3kxf coloured by chain, top view.
Source organisms:
Primary publication:
Hard wiring of T cell receptor specificity for the major histocompatibility complex is underpinned by TCR adaptability.
Burrows SR, Chen Z, Archbold JK, Tynan FE, Beddoe T, Kjer-Nielsen L, Miles JJ, Khanna R, Moss DJ, Liu YC, Gras S, Kostenko L, Brennan RM, Clements CS, Brooks AG, Purcell AW, McCluskey J, Rossjohn J
Proc Natl Acad Sci U S A 107 10608-13 (2010)
PMID: 20483993

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-498017 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, B alpha chain Chains: A, C, I, K
Molecule details ›
Chains: A, C, I, K
Length: 276 amino acids
Theoretical weight: 31.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01889 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, F, J, L
Molecule details ›
Chains: B, F, J, L
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
T cell receptor alpha chain constant Chains: D, G, M, N
Molecule details ›
Chains: D, G, M, N
Length: 204 amino acids
Theoretical weight: 23.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01848 (Residues: 1-89; Coverage: 64%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
T cell receptor beta constant 2 Chains: E, H, O, P
Molecule details ›
Chains: E, H, O, P
Length: 241 amino acids
Theoretical weight: 27.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
Gene names: TCRBC2, TRBC2
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Lytic switch protein BZLF1 Chains: Q, R, S, T
Molecule details ›
Chains: Q, R, S, T
Length: 13 amino acids
Theoretical weight: 1.43 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
UniProt:
  • Canonical: P03206 (Residues: 52-64; Coverage: 5%)
Gene name: BZLF1

Ligands and Environments

1 bound ligand:
Ligand IOD 24 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 78.69Å b: 207.077Å c: 123.512Å
α: 90° β: 90.19° γ: 90°
R-values:
R R work R free
0.217 0.213 0.291
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

24 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
23 more

5 mentions without citation

Hard wiring of T cell receptor specificity for the major histocompatibility complex is underpinned by TCR adaptability.
Burrows et al. (2010)
4 more