› 3l5x

X-ray diffraction
1.9Å resolution

Crystal structure of the complex between IL-13 and H2L6 FAB

Released:
DOI: 10.2210/pdb3l5x/pdb
PDB entry 3l5x coloured by chain, front view.
PDB entry 3l5x coloured by chain, side view.
PDB entry 3l5x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human framework adaptation of a mouse anti-human IL-13 antibody.
Fransson J, Teplyakov A, Raghunathan G, Chi E, Cordier W, Dinh T, Feng Y, Giles-Komar J, Gilliland G, Lollo B, Malia TJ, Nishioka W, Obmolova G, Zhao S, Zhao Y, Swanson RV, Almagro JC
J Mol Biol 398 214-31 (2010)
PMID: 20226193

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-543258 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H2L6 LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
H2L6 HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 224 amino acids
Theoretical weight: 24.11 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Interleukin-13 Chain: A
Molecule details ›
Chain: A
Length: 113 amino acids
Theoretical weight: 12.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35225 (Residues: 35-146; Coverage: 92%)
Gene names: IL13, NC30
Sequence domains: Interleukin-13
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

2 bound ligands:
Ligand MES 1 x MES
Ligand GOL 1 x GOL
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 63.78Å b: 73.02Å c: 114.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.197 0.247
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

2 review citations

Progress and Challenges in the Design and Clinical Development of Antibodies for Cancer Therapy.
Almagro et al. (2017)
1 more

8 mentions without citation

Computationally-driven identification of antibody epitopes.
Hua et al. (2017)
7 more