› 3lk4

X-ray diffraction
1.99Å resolution

Crystal structure of CapZ bound to the uncapping motif from CD2AP

Released:
DOI: 10.2210/pdb3lk4/pdb
PDB entry 3lk4 coloured by chain, front view.
PDB entry 3lk4 coloured by chain, side view.
PDB entry 3lk4 coloured by chain, top view.
Source organisms:
Primary publication:
Structural characterization of a capping protein interaction motif defines a family of actin filament regulators.
Hernandez-Valladares M, Kim T, Kannan B, Tung A, Aguda AH, Larsson M, Cooper JA, Robinson RC
Nat Struct Mol Biol 17 497-503 (2010)
PMID: 20357771

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-146483 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
F-actin-capping protein subunit alpha-1 Chains: 1, 4, 7, A, D, G, J, M, P, S, V, Y
Molecule details ›
Chains: 1, 4, 7, A, D, G, J, M, P, S, V, Y
Length: 286 amino acids
Theoretical weight: 33 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P13127 (Residues: 1-286; Coverage: 100%)
Gene name: CAPZA1
Sequence domains: F-actin capping protein alpha subunit
Structure domains:
F-actin-capping protein subunit beta isoforms 1 and 2 Chains: 2, 5, 8, B, E, H, K, N, Q, T, W, Z
Molecule details ›
Chains: 2, 5, 8, B, E, H, K, N, Q, T, W, Z
Length: 277 amino acids
Theoretical weight: 31.4 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14315 (Residues: 1-277; Coverage: 100%)
Gene name: CAPZB
Sequence domains: F-actin capping protein, beta subunit
Structure domains:
CD2-associated protein Chains: 0, 3, 6, 9, C, F, I, L, O, R, U, X
Molecule details ›
Chains: 0, 3, 6, 9, C, F, I, L, O, R, U, X
Length: 29 amino acids
Theoretical weight: 3.22 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9Y5K6 (Residues: 475-503; Coverage: 5%)
Gene name: CD2AP

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P1
Unit cell:
a: 59.728Å b: 142.341Å c: 192.999Å
α: 90° β: 89.99° γ: 90°
R-values:
R R work R free
0.228 0.225 0.272
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

14 review citations

Capping protein regulators fine-tune actin assembly dynamics.
Edwards et al. (2014)
13 more

4 mentions without citation

Structural characterization of a capping protein interaction motif defines a family of actin filament regulators.
Hernandez-Valladares et al. (2010)
3 more