› 3lrd

X-ray diffraction
2.15Å resolution

Self-assembly of spider silk proteins is controlled by a pH-sensitive relay

Released:
DOI: 10.2210/pdb3lrd/pdb
PDB entry 3lrd coloured by chain, front view.
PDB entry 3lrd coloured by chain, side view.
PDB entry 3lrd coloured by chain, top view.
Source organism: Euprosthenops australis
Primary publication:
Self-assembly of spider silk proteins is controlled by a pH-sensitive relay.
Askarieh G, Hedhammar M, Nordling K, Saenz A, Casals C, Rising A, Johansson J, Knight SD
Nature 465 236-8 (2010)
PMID: 20463740

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spidroin N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 137 amino acids
Theoretical weight: 14.18 KDa
Source organism: Euprosthenops australis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05H60 (Residues: 24-156; Coverage: 35%)
Gene name: MaSp1
Sequence domains: Major ampullate spidroin 1, spider silk protein 1, N-term
Structure domains: Enzyme I; Chain A, domain 2

Ligands and Environments

2 bound ligands:
Ligand EDO 2 x EDO
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3221
Unit cell:
a: 68.383Å b: 68.383Å c: 97.781Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.17 0.228
Expression system: Escherichia coli

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Citations

41 review citations

New opportunities for an ancient material.
Omenetto et al. (2010)
40 more