› 3mfb

X-ray diffraction
2.2Å resolution

Crystal Structure of the S-type Pyocin domain of ECA1669 protein from Erwinia carotovora, Northeast Structural Genomics Consortium Target EwR82C

Released:
DOI: 10.2210/pdb3mfb/pdb
PDB entry 3mfb coloured by chain, front view.
PDB entry 3mfb coloured by chain, side view.
PDB entry 3mfb coloured by chain, top view.
Source organism: Pectobacterium atrosepticum
Entry authors: Forouhar F, Neely H, Seetharaman J, Sahdev S, Xiao R, Ciccosanti C, Lee D, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179500 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyosin/cloacin translocation domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 157 amino acids
Theoretical weight: 18.31 KDa
Source organism: Pectobacterium atrosepticum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6D6L3 (Residues: 332-479; Coverage: 26%)
Gene name: ECA1669
Sequence domains: S-type Pyocin

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P1
Unit cell:
a: 50.076Å b: 52.307Å c: 62.161Å
α: 69.5° β: 90.04° γ: 90.04°
R-values:
R R work R free
0.236 0.232 0.265
Expression system: Escherichia coli BL21(DE3)

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