› 3mjh

X-ray diffraction
2.03Å resolution

Crystal Structure of Human Rab5A in complex with the C2H2 Zinc Finger of EEA1

Released:
DOI: 10.2210/pdb3mjh/pdb
PDB entry 3mjh coloured by chain, front view.
PDB entry 3mjh coloured by chain, side view.
PDB entry 3mjh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for Rab GTPase recognition and endosome tethering by the C2H2 zinc finger of Early Endosomal Autoantigen 1 (EEA1).
Mishra A, Eathiraj S, Corvera S, Lambright DG
Proc Natl Acad Sci U S A 107 10866-71 (2010)
PMID: 20534488

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148946 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rab-5A Chains: A, C
Molecule details ›
Chains: A, C
Length: 168 amino acids
Theoretical weight: 18.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20339 (Residues: 16-183; Coverage: 78%)
Gene names: RAB5, RAB5A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Early endosome antigen 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 34 amino acids
Theoretical weight: 3.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15075 (Residues: 36-69; Coverage: 2%)
Gene names: EEA1, ZFYVE2

Ligands and Environments

3 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 46.412Å b: 80.398Å c: 103.495Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.26
Expression system: Escherichia coli

Quick links

Citations

14 review citations

The cell biology of phagocytosis.
Flannagan et al. (2012)
13 more

18 mentions without citation

Rab GTPases and their interacting protein partners: Structural insights into Rab functional diversity.
Pylypenko et al. (2018)
17 more