› 3mn0

X-ray diffraction
1.65Å resolution

Introducing a 2-His-1-Glu Non-Heme Iron Center into Myoglobin confers Nitric Oxide Reductase activity: Cu(II)-CN-FeBMb(-His) form

Released:
DOI: 10.2210/pdb3mn0/pdb
PDB entry 3mn0 coloured by chain, front view.
PDB entry 3mn0 coloured by chain, side view.
PDB entry 3mn0 coloured by chain, top view.
Source organism: Physeter catodon
Primary publication:
Introducing a 2-His-1-Glu nonheme iron center into myoglobin confers nitric oxide reductase activity.
Lin YW, Yeung N, Gao YG, Miner KD, Lei L, Robinson H, Lu Y
J Am Chem Soc 132 9970-2 (2010)
PMID: 20586490

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 17.24 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02185 (Residues: 2-154; Coverage: 99%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

3 bound ligands:
Ligand HEM 1 x HEM
Ligand CU 1 x CU
Ligand CYN 1 x CYN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 39.61Å b: 48.42Å c: 77.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Design and engineering of artificial oxygen-activating metalloenzymes.
Nastri et al. (2016)
6 more

6 mentions without citation

Introducing a 2-His-1-Glu nonheme iron center into myoglobin confers nitric oxide reductase activity.
Lin et al. (2010)
5 more