› 3mp8

X-ray diffraction
1.92Å resolution

Crystal structure of Sgf29 tudor domain

Released:
DOI: 10.2210/pdb3mp8/pdb
Model geometry
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PDB entry 3mp8 coloured by chain, front view.
PDB entry 3mp8 coloured by chain, side view.
PDB entry 3mp8 coloured by chain, top view.
Source organisms:
Primary publication:
Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation.
Bian C, Xu C, Ruan J, Lee KK, Burke TL, Tempel W, Barsyte D, Li J, Wu M, Zhou BO, Fleharty BE, Paulson A, Allali-Hassani A, Zhou JQ, Mer G, Grant PA, Workman JL, Zang J, Min J
EMBO J 30 2829-42 (2011)
PMID: 21685874

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142525 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; SAGA-associated factor 29 Chain: A
Molecule details ›
Chain: A
Length: 522 amino acids
Theoretical weight: 57.52 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: P25554 (Residues: 105-259; Coverage: 60%)
Gene names: JW3994, SGF29, YCL010C, YCL10C, b4034, malE
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
5 bound ligands:
Ligand GOL 9 x GOL
Ligand SO4 6 x SO4
Ligand NA 5 x NA
Ligand ACY 3 x ACY
Ligand 4BZ 1 x 4BZ
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 49.76Å b: 95.1Å c: 114.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.216
Expression system: Escherichia coli BL21(DE3)

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Citations

51 review citations

Perceiving the epigenetic landscape through histone readers.
Musselman et al. (2012)
50 more

2 mentions without citation

Crystal structures of MBP fusion proteins.
Waugh DS. (2016)
1 more

PDB-REDO

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