› 3mxp

X-ray diffraction
1.61Å resolution

Crystal structure of Staphylococcal nuclease variant Delta+PHS T62A at cryogenic temperature

Released:
DOI: 10.2210/pdb3mxp/pdb
PDB entry 3mxp coloured by chain, front view.
PDB entry 3mxp coloured by chain, side view.
PDB entry 3mxp coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Cavities determine the pressure unfolding of proteins.
Roche J, Caro JA, Norberto DR, Barthe P, Roumestand C, Schlessman JL, Garcia AE, García-Moreno BE, Royer CA
Proc Natl Acad Sci U S A 109 6945-50 (2012)
PMID: 22496593

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-523735 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.11 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00644 (Residues: 83-231; Coverage: 70%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
Ligand THP 1 x THP
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 31.043Å b: 60.498Å c: 38.059Å
α: 90° β: 93.01° γ: 90°
R-values:
R R work R free
0.196 0.196 0.247
Expression system: Escherichia coli

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Citations

25 review citations

Water Determines the Structure and Dynamics of Proteins.
Bellissent-Funel et al. (2016)
24 more

1 mention without citation

Cavities determine the pressure unfolding of proteins.
Roche et al. (2012)