› 3n0l

X-ray diffraction
1.8Å resolution

Crystal structure of serine hydroxymethyltransferase from Campylobacter jejuni

Released:
DOI: 10.2210/pdb3n0l/pdb
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PDB entry 3n0l coloured by chain, front view.
PDB entry 3n0l coloured by chain, side view.
PDB entry 3n0l coloured by chain, top view.
Source organism: Campylobacter jejuni
Entry authors: Anderson SM, Wawrzak Z, Onopriyenko O, Hasseman J, Anderson WF, Savchenko A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150243 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 417 amino acids
Theoretical weight: 46.72 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24531 (Residues: 1-414; Coverage: 100%)
Gene names: Cj0402, glyA
Sequence domains: Serine hydroxymethyltransferase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 15 x SO4
1 modified residue:
Ligand MSE 26 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 58.558Å b: 108.319Å c: 126.072Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.198
Expression system: Escherichia coli BL21(DE3)

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Experimental raw data

Links to raw experimental data available for this entry are listed below

Diffraction data related to PDB entry 3n0l found at the Integrated Resource for Reproducibility in Macromolecular Crystallography (IRRMC)

Data DOI: 10.18430/m33n0l
Datasets: 2
Total size: 3.5Gb
Protein diffraction thumbnail