› 3nsn

X-ray diffraction
2.1Å resolution

Crystal Structure of insect beta-N-acetyl-D-hexosaminidase OfHex1 complexed with TMG-chitotriomycin

Released:
DOI: 10.2210/pdb3nsn/pdb
PDB entry 3nsn coloured by chain, front view.
PDB entry 3nsn coloured by chain, side view.
PDB entry 3nsn coloured by chain, top view.
Source organism: Ostrinia furnacalis
Primary publication:
Structural determinants of an insect beta-N-Acetyl-D-hexosaminidase specialized as a chitinolytic enzyme.
Liu T, Zhang H, Liu F, Wu Q, Shen X, Yang Q
J Biol Chem 286 4049-58 (2011)
PMID: 21106526

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitooligosaccharidolytic beta-N-acetylglucosaminidase Chain: A
Molecule details ›
Chain: A
Length: 572 amino acids
Theoretical weight: 65.55 KDa
Source organism: Ostrinia furnacalis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q06GJ0 (Residues: 23-594; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, TMX
Ligand NAG 3 x NAG
Ligand TMX 1 x TMX
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3221
Unit cell:
a: 108.5Å b: 108.5Å c: 175.57Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.202 not available
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Biosynthesis, Turnover, and Functions of Chitin in Insects.
Zhu et al. (2016)
32 other articles

10 mentions without citation

Review on Structures of Pesticide Targets.
Li et al. (2020)
9 more