› 3ntx

X-ray diffraction
1.9Å resolution

Crystal Structure of L-asparaginase I from Yersinia pestis

Released:
DOI: 10.2210/pdb3ntx/pdb
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PDB entry 3ntx coloured by chain, side view.
PDB entry 3ntx coloured by chain, top view.
Source organism: Yersinia pestis CO92
Entry authors: Kim Y, Gu M, Hasseman J, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104901 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
asparaginase Chains: A, B
Molecule details ›
Chains: A, B
Length: 341 amino acids
Theoretical weight: 37.68 KDa
Source organism: Yersinia pestis CO92
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A3N4B0Q2 (Residues: 1-338; Coverage: 100%)
Gene names: YPO2161, ansA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 125.377Å b: 109.089Å c: 58.224Å
α: 90° β: 117.63° γ: 90°
R-values:
R R work R free
0.156 0.154 0.192
Expression system: Escherichia coli BL21

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Experimental raw data

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Diffraction data related to PDB entry 3ntx found at the Integrated Resource for Reproducibility in Macromolecular Crystallography (IRRMC)

Data DOI: 10.18430/m33ntx
Datasets: 1
Total size: 1.46Gb
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