› 3nv0

X-ray diffraction
1.84Å resolution

Crystal structure and mutational analysis of the NXF2/NXT1 heterodimeric complex from caenorhabditis elegans at 1.84 A resolution

Released:
DOI: 10.2210/pdb3nv0/pdb
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PDB entry 3nv0 coloured by chain, front view.
PDB entry 3nv0 coloured by chain, side view.
PDB entry 3nv0 coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
The structure of the NXF2/NXT1 heterodimeric complex reveals the combined specificity and versatility of the NTF2-like fold.
Kerkow DE, Carmel AB, Menichelli E, Ambrus G, Hills RD, Gerace L, Williamson JR
J Mol Biol 415 649-65 (2012)
PMID: 22123199

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193625 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclear RNA export factor 2 Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 23.53 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9XVS8 (Residues: 202-405; Coverage: 50%)
Gene names: C15H11.6, nfx-2, nxf-2
Sequence domains: Nuclear RNA export factor, NTF2 domain
Structure domains: Nuclear Transport Factor 2; Chain: A,
NTF2-related export protein Chain: B
Molecule details ›
Chain: B
Length: 154 amino acids
Theoretical weight: 17.36 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9U757 (Residues: 2-137; Coverage: 99%)
Gene names: Y71F9AM.5, nxt-1, nxt1
Sequence domains: Nuclear transport factor 2 (NTF2) domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

4 bound ligands:
Ligand PEG 5 x PEG
Ligand EDO 1 x EDO
Ligand NA 19 x NA
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 42.004Å b: 49.547Å c: 148.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.191
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Integration of mRNP formation and export.
Björk et al. (2017)
2 more

2 mentions without citation

Domain organization within the nuclear export factor Mex67:Mtr2 generates an extended mRNA binding surface.
Aibara et al. (2015)
1 more

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