› 3o03

X-ray diffraction
1.9Å resolution

Quaternary complex structure of gluconate 5-dehydrogenase from streptococcus suis type 2

Released:
DOI: 10.2210/pdb3o03/pdb
PDB entry 3o03 coloured by chain, front view.
PDB entry 3o03 coloured by chain, side view.
PDB entry 3o03 coloured by chain, top view.
Source organism: Streptococcus suis 05ZYH33
Primary publication:
Structural insight into the catalytic mechanism of gluconate 5-dehydrogenase from Streptococcus suis: Crystal structures of the substrate-free and quaternary complex enzymes.
Zhang Q, Peng H, Gao F, Liu Y, Cheng H, Thompson J, Gao GF
Protein Sci 18 294-303 (2009)
PMID: 19177572

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-237229 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dehydrogenase with different specificities Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 31.06 KDa
Source organism: Streptococcus suis 05ZYH33
Expression system: Escherichia coli
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand NAP 1 x NAP
Ligand GCO 1 x GCO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: I222
Unit cell:
a: 71.244Å b: 75.272Å c: 98.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.242
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Uncovering newly emerging variants of Streptococcus suis, an important zoonotic agent.
Feng et al. (2010)
11 other articles

3 mentions without citation

Metabolic fate of unsaturated glucuronic/iduronic acids from glycosaminoglycans: molecular identification and structure determination of streptococcal isomerase and dehydrogenase.
Maruyama et al. (2015)
2 more