› 3ods

X-ray diffraction
1.9Å resolution

Crystal structure of the K185A mutant of the N-terminal domain of human Symplekin

Released:
DOI: 10.2210/pdb3ods/pdb
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PDB entry 3ods coloured by chain, front view.
PDB entry 3ods coloured by chain, side view.
PDB entry 3ods coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human symplekin-Ssu72-CTD phosphopeptide complex.
Xiang K, Nagaike T, Xiang S, Kilic T, Beh MM, Manley JL, Tong L
OpenAccess logo Nature 467 729-33 (2010)
PMID: 20861839

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187757 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Symplekin Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 46.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92797 (Residues: 1-395; Coverage: 31%)
Gene names: SPK, SYMPK
Sequence domains: Symplekin/PTA1 N-terminal
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 32.193Å b: 44.039Å c: 63.463Å
α: 79.45° β: 80.55° γ: 73.84°
R-values:
R R work R free
0.179 0.176 0.231
Expression system: Escherichia coli

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Citations

39 review citations

Mechanisms and consequences of alternative polyadenylation.
Di Giammartino et al. (2011)
38 more

6 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
5 more

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