› 3pgf

X-ray diffraction
2.1Å resolution

Crystal structure of maltose bound MBP with a conformationally specific synthetic antigen binder (sAB)

Released:
DOI: 10.2210/pdb3pgf/pdb
PDB entry 3pgf coloured by chain, front view.
PDB entry 3pgf coloured by chain, side view.
PDB entry 3pgf coloured by chain, top view.
Source organisms:
Primary publication:
Allosteric control of ligand-binding affinity using engineered conformation-specific effector proteins.
Rizk SS, Paduch M, Heithaus JH, Duguid EM, Sandstrom A, Kossiakoff AA
OpenAccess logo Nat Struct Mol Biol 18 437-42 (2011)
PMID: 21378967

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-142615 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 398 amino acids
Theoretical weight: 44.18 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-393; Coverage: 99%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II
Immunoglobulin gamma-1 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 231 amino acids
Theoretical weight: 24.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DOX5 (Residues: 1-106, 107-227; Coverage: 50%)
Sequence domains:
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TCD0 (Residues: 20-239; Coverage: 98%)
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand IMD 2 x IMD
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 63.87Å b: 228.46Å c: 135.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.227
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

6 review citations

Antibody-enabled small-molecule drug discovery.
Lawson AD. (2012)
5 more

13 mentions without citation

Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain.
Li et al. (2014)
12 more